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Protocols

 

Protocol: Description and Illustration 

Download/view protocol

1- Protocol for labeling the cell membranes and metaphase spindle poles of Ascidian embryos 

2- Imaris Protocol - Manual segmentation of multi-dimensional image .

This segmentation creates a 3D object from a multidimensional image.

 

  

 

 

3- Imaris protocol - Extraction of the apical surface of a cell previously modeled 3D. 

 

 

 

 

4- Matlab protocol - Determination of the long axis of the blastomers according to the mathematical model of Minc:

 

- The script determines the distance between the central position of the predicted mitotic spindle and the central position of the observed mitotic spindle ("Centering deviation").

 

- The script determines the angle difference between the predicted mitotic spindle and the observed mitotic spindle ("Orienting deviation").

 

 

Protocols related to the project Fertilization and Meïosis

Protocols related to the project Marine embryoTox

12/10/17

Traductions :

 

 Alex McDougall

  

Rémi Dumollard

 

Ievgeniia Gazo

 

Isa Gomes

 

 Bioclips

Fertilization

Meiosis

 

 

06/10/17